Nicotine has an excitatory effect on the resting potential of neurons. When nicotine binds to nicotinic acetylcholine receptors on the postsynaptic membrane, it opens ion channels, allowing an influx of positively charged ions, such as sodium and calcium, into the cell. This influx of ions depolarizes the cell, bringing it closer to the threshold for action potential generation. As a result, the resting potential becomes less negative, making the neuron more likely to fire an action potential in response to a stimulus. **

The amount of nicotine liquid needed for nicotine shots depends on the desired nicotine strength of the final e-liquid. Typically, a 10ml bottle of nicotine liquid with a concentration of 18mg/ml is used to make a 60ml bottle of e-liquid with a nicotine strength of 3mg/ml. This means that for every 60ml of e-liquid, 10ml of 18mg/ml nicotine liquid is needed to achieve the desired nicotine strength. It's important to carefully measure and mix the nicotine liquid with the base e-liquid to ensure the correct nicotine concentration. **

No, nicotine itself cannot mold. Mold typically grows on organic materials like food, wood, or paper that provide nutrients for mold spores to thrive. Nicotine is a chemical compound found in tobacco plants and is not a suitable environment for mold growth. However, if nicotine-containing products like cigarettes or vaping devices are exposed to moisture, they can potentially develop mold on the organic components of the product. **

Nicotine poisoning can occur at different levels depending on the individual's tolerance and sensitivity. However, symptoms of nicotine poisoning typically start to appear at doses of 1 milligram of nicotine per kilogram of body weight. This means that for an average adult, nicotine poisoning could occur at around 60-100 milligrams of nicotine. It is important to seek medical attention immediately if you suspect nicotine poisoning. **

Nicotine poisoning can occur from ingesting as little as 10 milligrams of nicotine, which is roughly equivalent to the amount found in one cigarette. Symptoms of nicotine poisoning can include nausea, vomiting, dizziness, increased heart rate, and in severe cases, seizures or respiratory failure. It is important to seek medical attention immediately if you suspect someone has nicotine poisoning. **

To add nicotine to a finished nicotine-free liquid, you will need to purchase a nicotine concentrate in the desired strength. You can then use a syringe or pipette to measure out the appropriate amount of nicotine concentrate and carefully add it to the finished liquid. It's important to mix the nicotine thoroughly with the liquid to ensure an even distribution. Be sure to wear gloves and handle the nicotine concentrate with caution, as it is a toxic substance. Always follow safety guidelines and keep nicotine products out of reach of children and pets. **

No, you cannot get a nicotine shock from using a vape without nicotine because nicotine is the substance responsible for causing nicotine shocks. Vapes without nicotine typically contain flavorings and other ingredients but not nicotine. However, it is still important to be cautious and aware of the potential risks associated with vaping, even without nicotine. **

A mixture of 50% nicotine and 70% base would contain 35% nicotine (50% of 70%). To calculate this, you would multiply the two percentages together. Therefore, the mixture would contain 35% nicotine. **